"Investigation of the Diagnostic Accuracy of Rapid Influenza Diagnostic Kit Method in Comparison to the Real Time Reverse Transcription Polymerase Chain Reaction"
نویسندگان
چکیده
Bimalesh Kumar Jha1*, Rachana Mehta1 and Krishna Das manandhar2 Author Affiliations 1National Public Health Laboratory, Nepal 2Central Department of Biotechnology, Tribhuvan University, Received: August 26, 2022 | Published: September 23, Corresponding author: Jha, National Teku, Kathmandu, DOI: 10.26717/BJSTR.2022.46.007341
منابع مشابه
A diagnostic one-step real-time reverse transcription polymerase chain reaction method for accurate detection of influenza virus type A
INTRODUCTION Influenza A is known as a public health concern worldwide. In this study, a novel one-step real-time reverse transcription polymerase chain reaction (rtRT-PCR) assay was designed and optimized for the detection of influenza A viruses. MATERIAL AND METHODS The primers and probe were designed based on the analysis of 90 matrix nucleotide sequence data of influenza type A subtypes f...
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Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome generates robust data that are informative and, most importantly, generally applicable. This is because the information contained within the genome is context-independent; i.e., generally, every normal cell contains the same DNA sequence--the same mutations and polymo...
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ژورنال
عنوان ژورنال: Biomedical Journal of Scientific and Technical Research
سال: 2022
ISSN: ['2574-1241']
DOI: https://doi.org/10.26717/bjstr.2022.46.007341